top of page
Dr Mark Osborne
science > ideas > solutions
Create Your First Project
Start adding your projects to your portfolio. Click on "Manage Projects" to get started
RSC: Single Molecule Resolution by DORIS
Reference
Boulineau, R. L. & Osborne, M. A. Direct object resolution by image subtraction: A new molecular ruler for nanometric measurements on complexed fluorophores. Chem. Commun. 49, 5559–5561 (2013)
A technique for measuring distances between two or more fluorophores spaced in the 10–100 nm range is described. We identify a linear correlation between the intensity–amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.
bottom of page